ULBP-2 Protein, Human, Recombinant (His)

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Catalog No. TMPY-02333

ULBP-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.2 kDa and the accession number is Q9BZM5.

ULBP-2 Protein, Human, Recombinant (His)

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Catalog No. TMPY-02333

ULBP-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.2 kDa and the accession number is Q9BZM5.

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$48	7-10 days	7-10 days
10 μg	$75	7-10 days	7-10 days
20 μg	$118	7-10 days	7-10 days
50 μg	$229	7-10 days	7-10 days
100 μg	$386	-	In Stock
200 μg	$682	7-10 days	7-10 days
500 μg	$1,430	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Product Information

Biological Activity	Loaded Recombinant Human ULBP2 Protein, His Tag (Cat#TMPY-02333) on NTA Biosensor, can bind Recombinant Human NKG2D Protein, mFc Tag (Cat#TMPY-06827) with an affinity constant of 0.247 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
Description	ULBP-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.2 kDa and the accession number is Q9BZM5.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9BZM5
Synonyms	UNQ463 / PRO791,UL16 binding protein 2,RAET1H,NKG2DL2,N2DL2,ALCAN-α,ALCAN-alpha
Construction	A DNA sequence encoding the human ULBP2 (Q9BZM5) (Met 1-Ser 217), without the pro peptide, was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gly 26
Protein Purity	> 97 % as determined by SDS-PAGE
Molecular Weight	23.2 kDa (predicted); 33 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	NKG2D ligand 2, also known as N2DL-2, NKG2DL2, ALCAN-alpha, Retinoic acid early transcript 1H, UL16-binding protein 2, ULBP2 and N2DL2, is cell membrane protein that belongs to the MHC class I family. ULBP2 / N2DL-2 is expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. ULBP2 / N2DL-2 is a ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway.