Thyroid Peroxidase Protein, Mouse, Recombinant (His)

Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 465.00
500 μg	5 days	$ 1,850.00
1 mg	5 days	$ 3,100.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 465.00

500 μg

5 days

$ 1,850.00

1 mg

5 days

$ 3,100.00

Description

Species	Mouse
Expression System	HEK293
Tag	C-His
Accession Number	P35419
Synonyms	MK-CSF, MGDF, ML, MPLLG, THPO, TPOMKCSF, Thrombopoietin, MKCSF, MGDFC-mpl ligand, THCYT1, TPO, MGC163194, MPL ligand
Construction	Recombinant Mouse Thyroid Peroxidase Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly32-Arg834.[Accession \|P35419]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 90.6 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase that encodes a 933 amino-acid residue (aa) molecule with a single membrane-spanning region. Thyroid peroxidase plays a key role in thyroid hormone synthesis by catalyzing both the iodination of tyrosine residues to form monoiodotyrosine (MIT) and diiodotyrosine (DIT) residues and the coupling of iodotyrosine residues in Tg, resulting in the formation of T3 and T4. It is a frequent epitope of autoantibodies in autoimmune thyroid disease, for example, the expression of thyroid peroxidase is lost in papillary thyroid carcinoma.