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Osteopontin Protein, Mouse, Recombinant (His)
(Synonyms: Uropontin, SPP-1, SPP1, Osteopontin, OPN, Nephropontin, ETA-1, BSPI, BSP-1, Bone sialoprotein 1, BNSP) Copy Product InfoSynonyms: Uropontin, SPP-1, SPP1, Osteopontin, OPN, Nephropontin, ETA-1, BSPI, BSP-1, Bone sialoprotein 1, BNSP
Catalog No. TMPK-00807 Copy Product Info
Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | - | In Stock | |
| 10 μg | $108 | 7-10 days | 7-10 days | |
| 20 μg | $177 | 7-10 days | 7-10 days | |
| 50 μg | $346 | 7-10 days | 7-10 days | |
| 100 μg | $581 | In Stock | In Stock | |
| 200 μg | $1,050 | 7-10 days | 7-10 days | |
| 500 μg | $2,330 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P10923 |
| Construction | Leu17-Asn294 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC   |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Uropontin, SPP-1, SPP1, Osteopontin, OPN, Nephropontin, ETA-1, BSPI, BSP-1, Bone sialoprotein 1, BNSP |
| Research Background |
| Molecular Weight | 31.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Osteopontin Protein, Mouse, Recombinant (His) chemical structure | Osteopontin Protein, Mouse, Recombinant (His) molecular weight
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