SEMA3A Protein, Mouse, Recombinant (His)

Catalog No. TMPY-04625 Copy Product Info

SEMA3A Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is A0A803Z7F8.

SEMA3A Protein, Mouse, Recombinant (His)

Catalog No. TMPY-04625

Copy Product Info

SEMA3A Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is A0A803Z7F8.

Pack Size	Price	USA Stock	Global Stock
5 μg	$100	7-10 days	7-10 days
10 μg	$163	7-10 days	7-10 days
20 μg	$269	7-10 days	7-10 days
50 μg	$536	7-10 days	7-10 days
100 μg	$904	-	In Stock

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SEMA3A Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is A0A803Z7F8.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His
Accession Number	O08665
Synonyms	SemD,Semad,SEMA1,sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A,Hsema-I,coll-1
Construction	A DNA sequence encoding the mouse SEMA3A (NP_001230001.1) (Lys26-Phe546) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	61.6 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 100 mM Argine, 100 mM glutamic acid, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease