FGF-8b Protein, Human, Recombinant

Name: FGF-8b Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-06986
Price: 59 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: NEWENTRY, KAL6, HH6, HBGF-8, fibroblast growth factor 8 (androgen-induced), FGF-8, AIGF) Copy Product Info

Synonyms: NEWENTRY, KAL6, HH6, HBGF-8, fibroblast growth factor 8 (androgen-induced), FGF-8, AIGF

Catalog No. TMPY-06986 Copy Product Info

In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.

Pack Size	Price	USA Stock	Global Stock
5 µg	$59	-	In Stock
10 µg	$94	-	In Stock
20 µg	$152	-	In Stock
50 µg	$297	-	In Stock
100 µg	$506	7-10 days	7-10 days
200 µg	$860	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:94.3%

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.4-1.6 μg/mL.
Description	In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P55075-3
Construction	A DNA sequence encoding the human FGF8b (P55075-3) (Gln23-Arg215) was expressed with an initial Met.
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. . Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	NEWENTRY, KAL6, HH6, HBGF-8, fibroblast growth factor 8 (androgen-induced), FGF-8, AIGF
Research Background	In mammalian embryos, transient Fgf8 expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. Retinoic acid (RA) directly represses Fgf8 through a RARE-mediated mechanism that promotes repressive chromatin, thus providing valuable insight into the mechanism of RA-FGF antagonism during progenitor cell differentiation. Fgf8 encodes a key signaling factor, and its precise regulation is essential for embryo patterning.

Chemical Properties

Molecular Weight

The recombinant human FGF8b consists 194 amino acids and predicts a molecular mass of 22.5 kDa.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.