PD-1 Protein, Mouse, Recombinant (His) V2

Catalog No. TMPY-00996U Copy Product Info

PD-1 Protein, Mouse, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is Q02242.

PD-1 Protein, Mouse, Recombinant (His) V2

Catalog No. TMPY-00996U

Copy Product Info

PD-1 Protein, Mouse, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is Q02242.

Pack Size	Price	USA Stock	Global Stock
5 μg	$31	7-10 days	7-10 days
10 μg	$46	7-10 days	7-10 days
20 μg	$71	7-10 days	7-10 days
50 μg	$133	7-10 days	7-10 days
100 μg	$220	7-10 days	7-10 days
200 μg	$400	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	Loaded Mouse PD-1-His on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested)
Description	PD-1 Protein, Mouse, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is Q02242.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	Q02242
Synonyms	programmed cell death 1,Pdc1,PD-1,Ly101
Construction	Leu25-Gln167
Protein Purity	> 95% as determined by SDS-PAGE
Molecular Weight	17.2 kDa (Predicted); 33-40 kDa (Reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Programmed Death-1 (PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1 (PD-1) with its ligands, programmed death ligand B7H1 (PD-L1) and B7DC (PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.