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PAM Protein, Human, Recombinant (His)

Catalog No. TMPY-00392
Synonyms: PAL, peptidylglycine alpha-amidating monooxygenase, peptidylglycine α-amidating monooxygenase, PHM

PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PAM Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 451.00
100 μg 5 days $ 771.00
200 μg 5 days $ 1,310.00
500 μg 5 days $ 2,660.00
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Biological Description
Technical Params
Product Properties
Biological Information Testing in progress
Description PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number P19021-2
Synonyms PAL, peptidylglycine alpha-amidating monooxygenase, peptidylglycine α-amidating monooxygenase, PHM
Construction The Human PAM (NP_620176.1) (Met1-Ser866) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Molecular Weight 94.4 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

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Keywords

PAM Protein, Human, Recombinant (His) PAL peptidylglycine alpha-amidating monooxygenase peptidylglycine a-amidating monooxygenase peptidylglycine α-amidating monooxygenase PHM recombinant recombinant-proteins proteins protein

 

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