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PAM Protein, Human, Recombinant (His)

Catalog No. TMPY-00392
Synonyms: PAL, peptidylglycine alpha-amidating monooxygenase, peptidylglycine α-amidating monooxygenase, PHM

Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PAM Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 451.00
100 μg 5 days $ 771.00
200 μg 5 days $ 1,310.00
500 μg 5 days $ 2,660.00
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Biological Description
Technical Params
Product Properties
Biological Information Testing in progress
Description Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.
Species Human
Expression System HEK293
Tag His
Accession Number P19021-2
Synonyms PAL, peptidylglycine alpha-amidating monooxygenase, peptidylglycine α-amidating monooxygenase, PHM
Construction A DNA sequence encoding the human PAM (NP_620176.1) (Met1-Ser866) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Molecular Weight Approxiamtely 94.4 kDa
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Formulation Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

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Keywords

PAM Protein, Human, Recombinant (His) PAL peptidylglycine alpha-amidating monooxygenase peptidylglycine a-amidating monooxygenase peptidylglycine α-amidating monooxygenase PHM recombinant recombinant-proteins proteins protein

 

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