PAM Protein, Human, Recombinant (His)

Name: PAM Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00392
Price: 86 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL) Copy Product Info

Synonyms: PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL

Catalog No. TMPY-00392 Copy Product Info

PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$86	-	In Stock
10 µg	$139	-	In Stock
20 µg	$229	-	In Stock
50 µg	$451	-	In Stock
100 µg	$771	7-10 days	7-10 days
200 µg	$1,310	7-10 days	7-10 days
500 µg	$2,660	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 92.6%; SEC-HPLC: 92.1%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 94.4 kDa and the accession number is P19021-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P19021-2
Construction	A DNA sequence encoding the human PAM (NP_620176.1) (Met1-Ser866) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Phe 31
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL
Research Background	Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

Chemical Properties

Molecular Weight

94.4 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.