PAM Protein, Human, Recombinant (hFc)

Name: PAM Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-00149
Rating: 4.5 (1 reviews)

(Synonyms: PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL) Copy Product Info

Synonyms: PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL

Catalog No. TMPY-00149 Copy Product Info

PAM Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 104 kDa and the accession number is P19021-2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	7-10 days	7-10 days
10 µg	$208	7-10 days	7-10 days
20 µg	$345	7-10 days	7-10 days
50 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PAM Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 104 kDa and the accession number is P19021-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P19021-2
Construction	A DNA sequence encoding the human PAM (NP_620176.1) (Met1-Val710) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 21
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	PHM, peptidylglycine α-amidating monooxygenase, peptidylglycine alpha-amidating monooxygenase, PAL
Research Background	Peptidylglycine alpha-amidating monooxygenase (PAM) is highly expressed in neurons and endocrine cells, where it catalyzes one of the final steps in the biosynthesis of bioactive peptides. PAM is also expressed in unicellular organisms such as Chlamydomonas reinhardtii, which do not store peptides in secretory granules. As for other granule membrane proteins, PAM is retrieved from the cell surface and returned to the trans-Golgi network. This pathway involves regulated entry of PAM into multivesicular body intralumenal vesicles (ILVs). Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides.

Chemical Properties

Molecular Weight

104 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.