Notch 2 Protein, Human, Recombinant (His & Avi)

Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 231.00
500 μg	5 days	$ 932.00
1 mg	5 days	$ 1,550.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 231.00

500 μg

5 days

$ 932.00

1 mg

5 days

$ 1,550.00

Description

Species	Human
Expression System	HEK293
Tag	C-His-Avi
Accession Number	Q04721
Synonyms	N2, Notch-2, HJCYS, AGS2, NOTCH2, N2ECD
Construction	Recombinant Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu26-GIn530.[Accession \|Q04721]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Stability & Storage	Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.