LIGHT/TNFSF14 Protein, Human, Recombinant (rFc)

Name: LIGHT/TNFSF14 Protein, Human, Recombinant (rFc)
Brand: TargetMol
SKU: TMPY-04833
Rating: 4.5 (1 reviews)

(Synonyms: tumor necrosis factor (ligand) superfamily, member 14, TR2, LTg, LIGHT, HVEML, CD258) Copy Product Info

Synonyms: tumor necrosis factor (ligand) superfamily, member 14, TR2, LTg, LIGHT, HVEML, CD258

Catalog No. TMPY-04833 Copy Product Info

LIGHT/TNFSF14 Protein, Human, Recombinant (rFc) is expressed in HEK293 mammalian cells with rFc tag. The predicted molecular weight is 45.7 kDa and the accession number is AAK26160.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$63	7-10 days	7-10 days
10 µg	$98	7-10 days	7-10 days
20 µg	$163	7-10 days	7-10 days
50 µg	$323	7-10 days	7-10 days
100 µg	$625	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25 ng/mL.
Description	LIGHT/TNFSF14 Protein, Human, Recombinant (rFc) is expressed in HEK293 mammalian cells with rFc tag. The predicted molecular weight is 45.7 kDa and the accession number is AAK26160.1.
Species	Human
Expression System	HEK293 Cells
Tag	N-rFc
Accession Number	AAK26160.1
Construction	A DNA sequence encoding the human TNFSF14 (AAK26160.1) (Asp74-Val240) was expressed with the Fc region of rabbit IgG at the N-terminus.. Predicted N terminal: Ser
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	tumor necrosis factor (ligand) superfamily, member 14, TR2, LTg, LIGHT, HVEML, CD258
Research Background	LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+and CD8+T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

45.7 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.