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Irisin Protein, Human/Mouse/Rat, Recombinant (N-His)

(Synonyms: Irisin, FNDC5, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5) Copy Product Info

Synonyms: Irisin, FNDC5, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5

Catalog No. TMPJ-01228 Copy Product Info
Irisin Protein, Human/Mouse/Rat, Recombinant (N-His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 18-28 KDa and the accession number is A0A0A0MRR6.
Irisin Protein, Human/Mouse/Rat, Recombinant (N-His)
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$80-In Stock
10 µg$129-In Stock
20 µg$198-In Stock
50 µg$390-In Stock
100 µg$626-In Stock
200 µg$9877-10 days7-10 days
500 µg$1,900-In Stock
1 mg$2,730-In Stock
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:SDS-PAGE: > 99.9%; SEC-HPLC: 99.13%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Irisin Protein, Human/Mouse/Rat, Recombinant (N-His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 18-28 KDa and the accession number is A0A0A0MRR6.
Species
Human,Mouse,Rat
Expression System
HEK293 Cells
TagN-6xHis
Accession NumberA0A0A0MRR6
Amino AcidAsp32-Glu143
ConstructionAsp32-Glu143
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Irisin Protein, Human/Mouse/Rat, Recombinant (N-His)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsIrisin, FNDC5, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5
Research Background
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Chemical Properties
Molecular Weight18-28 KDa (reducing condition)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: Irisin Protein, Human/Mouse/Rat, Recombinant (N-His) chemical structure | Irisin Protein, Human/Mouse/Rat, Recombinant (N-His) molecular weight