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Irisin Protein, Human/Mouse/Rat, Recombinant (C-His)

Catalog No. TMPJ-01227
Synonyms: FNDC5, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5, Irisin

Irisin Protein, Human/Mouse/Rat, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 20-28 KDa and the accession number is Q8NAU1.

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Irisin Protein, Human/Mouse/Rat, Recombinant (C-His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 129.00
50 μg 5 days $ 390.00
500 μg 5 days $ 1,900.00
1 mg 5 days $ 2,730.00
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Biological Description
Technical Params
Product Properties
Description Irisin Protein, Human/Mouse/Rat, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 20-28 KDa and the accession number is Q8NAU1.
Species Human,Mouse,Rat
Expression System HEK293 Cells
Tag C-6xHis
Accession Number Q8NAU1
Synonyms FNDC5, Fibronectin type III repeat-containing protein 2, Fibronectin type III domain-containing protein 5, Irisin
Amino Acid Asp32-Glu143
Construction Asp32-Glu143
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 20-28 KDa (reducing condition)
Endotoxin < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

Research Background Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

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Keywords

Irisin Protein, Human/Mouse/Rat, Recombinant (C-His) FNDC-5 FNDC 5 FNDC5 Fibronectin type III repeat-containing protein 2 Fibronectin type III domain-containing protein 5 Irisin recombinant recombinant-proteins proteins protein

 

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