IL-6 Protein, Rat, Recombinant

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
20 μg	In stock	$ 177.00
100 μg	5 days	$ 447.00
200 μg	5 days	$ 793.00
500 μg	5 days	$ 2,620.00

Pack Size

Availability

Price/USD

Quantity

20 μg

In stock

$ 177.00

100 μg

5 days

$ 447.00

200 μg

5 days

$ 793.00

500 μg

5 days

$ 2,620.00

Biological Information

Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml.

Description

Species	Rat
Expression System	E. coli
Tag	Tag Free
Accession Number	A6KJN3
Synonyms	interleukin 6
Construction	A DNA sequence encoding the rat IL6 (NP_036721.1)(Phe25-Thr211) was expressed and purified with an initial Met.
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 21.9 kDa

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile 40% acetonitrile, 0. 1% TFAPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy