Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 μM as determined in SPR assay (Biacore T200).

IL-1F10 Protein, Human, Recombinant (His) is expressed in yeast with His tag. The predicted molecular weight is 18.07 kDa and the accession number is AAK68048.1.

Human

P. pastoris (Yeast)

interleukin 1 family, member 10 (θ),interleukin 1 family, member 10 (theta),IL-38,IL1-θ,IL1-theta,IL-1HY2,IL1HY2,FKSG75,FIL1-θ,FIL1-theta

Met1-Trp152

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin (IL)-38, a newly discovered IL-1 family cytokine, is expressed in several tissues and secreted by various cells. IL-38 has recently been reported to exert an anti-inflammatory function by binding to several receptors, including interleukin-36 receptor (IL-36R), interleukin-1 receptor accessory protein-like 1 (IL-1RAPL1), and interleukin-1 receptor 1 (IL-1R1) to block binding with other pro-inflammatory cytokines and inhibit subsequent signaling pathways; thereby regulating the differentiation and function of T cells, peripheral blood mononuclear cells, macrophages, and dendritic cells.