GDF-15 Protein, Mouse, Recombinant (His)

Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 487.00
500 μg	5 days	$ 1,950.00
1 mg	5 days	$ 3,250.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 487.00

500 μg

5 days

$ 1,950.00

1 mg

5 days

$ 3,250.00

Biological Information

1. Immobilized Mouse GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.25μg/ml determined by ELISA (QC Test). 2. Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay.

Description

Species	Mouse
Expression System	E. coli
Tag	N-His
Accession Number	Q9Z0J7
Synonyms	MIC1, Placental TGF-beta, Placental TGF-β, PTGF-beta, PTGFBPTGF-β, MIC-1, PTGFB, RG-1, GDF-15, PTGFBPTGF-beta, PDF, NAG-1, PTGF-β, PLAB, GDF15
Construction	Recombinant Mouse GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser189-Ala303.[Accession \|Q9Z0J7]
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	The protein has a predicted MW of 13.78 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.