Fractalkine Protein, Human, Recombinant (His)

Fractalkine Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0N0N7.

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 306.00
1 mg	5 days	$ 3,060.00

Pack Size

Availability

Price/USD

Quantity

50 μg

5 days

$ 306.00

1 mg

5 days

$ 3,060.00

Description

Fractalkine Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0N0N7.

Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A0N0N7
Synonyms	CXC3, neurotactin, ABCD-3, CXC3C, chemokine (C-X3-C motif) ligand 1, fractalkine, C3Xkine, NTT, NTN, SCYD1
Construction	Human CX3CL1 (NP_002987.1) extracellular domain (Met 1-Arg 339) with a C-terminal polyhistidine tag
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	35 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.