FGF-1 Protein, Human, Recombinant V2

Catalog No. TMPY-00756U Copy Product Info

FGF-1 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P05230-1.

FGF-1 Protein, Human, Recombinant V2

Catalog No. TMPY-00756U

Copy Product Info

FGF-1 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P05230-1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$38	7-10 days	7-10 days
10 μg	$57	7-10 days	7-10 days
20 μg	$76	7-10 days	7-10 days
50 μg	$115	7-10 days	7-10 days
100 μg	$157	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.7 ng/mL (QC Test). Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
Description	FGF-1 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P05230-1.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P05230-1
Synonyms	HBGF-1,HBGF1,GLIO703,fibroblast growth factor 1 (acidic),FGF-α,FGF-alpha,FGFA,FGF-1,ECGF-β,ECGF-beta,ECGFB,ECGFA,ECGF,AFGF
Construction	Phe16-Asp155
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight	15.83 kDa (Predicted and reducing conditions)
Endotoxin	< 0.1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.