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Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI)

(Synonyms: Pseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA) Copy Product Info

Synonyms: Pseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA

Catalog No. TMPH-03168 Copy Product Info
Purity: 95%
An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38. Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) is expressed in E. coli expression system with N-6xHis-KSI tag. The predicted molecular weight is 39.9 kDa and the accession number is P11439.
Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI)
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$19820 days20 days
10 μg$339-In Stock
20 μg$570-In Stock
50 μg$86820 days20 days
100 μg$1,20020 days20 days
200 μg$1,69020 days20 days
500 μg$2,76020 days20 days
1 mg$3,97020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:95%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Description
An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38. Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) is expressed in E. coli expression system with N-6xHis-KSI tag. The predicted molecular weight is 39.9 kDa and the accession number is P11439.
Species
Pseudomonas aeruginosa
Expression System
E. coli
TagN-6xHis-KSI
Accession NumberP11439
Amino AcidALLERNYPTGAEFLGDGGDISFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGVRARSQDLDAIWRGFYIAGDPALAYGYAQDQEPDARGRIRNGALLRVYVPRSSLPGFYRTGLTLAAPEAAGEVERLIGHPLPLRLDAITGPEEEGGRLETILGWPLAERTVVIPSAIPTDPRNVGGDLDPSSIPDKEQAISALPDYASQPGKPPREDLK
Construction413-638 aa
Protein Purity
> 85% as determined by SDS-PAGE.
https://cdn.targetmol.com/group3/M00/53/4C/CgoaEWmEVPaEMSeaAAAAAO4keDE276.png
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsPseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA
Research Background
Chemical Properties
Molecular Weight39.9 kDa (predicted)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) chemical structure | Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) molecular weight