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Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI)

(Synonyms: Pseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA) Copy Product Info

Synonyms: Pseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA

Catalog No. TMPH-03168 Copy Product Info
Purity: 95%
An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38. Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) is expressed in E. coli expression system with N-6xHis-KSI tag. The predicted molecular weight is 39.9 kDa and the accession number is P11439.
Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$19820 days20 days
10 µg$339-In Stock
20 µg$570-In Stock
50 µg$86820 days20 days
100 µg$1,20020 days20 days
200 µg$1,69020 days20 days
500 µg$2,76020 days20 days
1 mg$3,97020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:95%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38. Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) is expressed in E. coli expression system with N-6xHis-KSI tag. The predicted molecular weight is 39.9 kDa and the accession number is P11439.
Species
Pseudomonas aeruginosa
Expression System
E. coli
TagN-6xHis-KSI
Accession NumberP11439
Amino AcidALLERNYPTGAEFLGDGGDISFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGVRARSQDLDAIWRGFYIAGDPALAYGYAQDQEPDARGRIRNGALLRVYVPRSSLPGFYRTGLTLAAPEAAGEVERLIGHPLPLRLDAITGPEEEGGRLETILGWPLAERTVVIPSAIPTDPRNVGGDLDPSSIPDKEQAISALPDYASQPGKPPREDLK
Construction413-638 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsPseudomonas exotoxin (PE), NAD(+)--diphthamide ADP-ribosyltransferase, Exotoxin A, ETA
Research Background
An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.
Chemical Properties
Molecular Weight39.9 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) chemical structure | Exotoxin A Protein, Pseudomonas aeruginosa, Recombinant (His & KSI) molecular weight