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ECHS1 Protein, Mouse, Recombinant (C225S, HA & His)

Catalog No. TMPH-02642
Synonyms: mECH, Enoyl-CoA hydratase, mitochondrial, SCEH, mECH1, ECHS1

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.

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ECHS1 Protein, Mouse, Recombinant (C225S, HA & His)
Pack Size Availability Price/USD Quantity
20 μg 20 days $ 491.00
100 μg 20 days $ 1,370.00
1 mg 20 days $ 2,750.00
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Biological Description
Technical Params
Product Properties
Description Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Species Mouse
Expression System Baculovirus
Tag N-terminal 10xHis-tagged and C-terminal HA-tagged
Accession Number Q8BH95
Synonyms mECH, Enoyl-CoA hydratase, mitochondrial, SCEH, mECH1, ECHS1
Amino Acid ASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVEKLVEEAIQSAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFVEKRKANFKDH Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Construction 28-290 aa (C225S)
Protein Purity > 85% as determined by SDS-PAGE.
Molecular Weight 32.0 kDa as predicted
Formulation If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.

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Keywords

ECHS1 Protein, Mouse, Recombinant (C225S, HA & His) mECH Enoyl-CoA hydratase, mitochondrial SCEH mECH1 ECHS1 recombinant recombinant-proteins proteins protein

 

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