CDK1 Protein, Mouse, Recombinant (His & GST)

Name: CDK1 Protein, Mouse, Recombinant (His & GST)
Brand: TargetMol
SKU: TMPY-04775
Rating: 4.5 (1 reviews)

(Synonyms: p34&lt, cyclin-dependent kinase 1, Cdc2a, CDC2&gt, Cdc2) Copy Product Info

Synonyms: p34&lt, cyclin-dependent kinase 1, Cdc2a, CDC2&gt, Cdc2

Catalog No. TMPY-04775 Copy Product Info

CDK1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.9 kDa and the accession number is P11440.

Pack Size	Price	USA Stock	Global Stock
10 µg	$93	7-10 days	7-10 days
20 µg	$152	7-10 days	7-10 days
50 µg	$343	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Kinase activity untested
Description	CDK1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.9 kDa and the accession number is P11440.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P11440
Construction	A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 85 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM Nacl, 10% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	p34&lt, cyclin-dependent kinase 1, Cdc2a, CDC2&gt, Cdc2
Research Background	CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

Chemical Properties

Molecular Weight

61.9 kDa (predicted); 57 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.