CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 109.7 kDa. Accession number: NP_001777.1&NP_001229.1
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 498.00 |
Description | CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 109.7 kDa. Accession number: NP_001777.1&NP_001229.1 |
Species | Human |
Expression System | Baculovirus-Insect Cells |
Tag | His,GST |
Accession Number | NP_001777.1&NP_001229.1 |
Synonyms | P34CDC2 & CCNE1, CDC2, CDKN1, CDK1, CCNE, CDC28A |
Construction | A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified. |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | Approxiamtely 109.7 kDa |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Formulation | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) P34CDC2 & CCNE1 CDKN-1 CDC 2 CDK 1 CDC2 CDC-2 CDK-1 CDKN1 CDK1 CCNE CDKN 1 CDC28A recombinant recombinant-proteins proteins protein