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CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04450
Synonyms: P34CDC2 & CCNE1, CDC2, CDKN1, CDK1, CCNE, CDC28A

CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 109.7 kDa. Accession number: NP_001777.1&NP_001229.1

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST)
Pack Size Availability Price/USD Quantity
50 μg 5 days $ 498.00
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Biological Description
Technical Params
Product Properties
Description CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 109.7 kDa. Accession number: NP_001777.1&NP_001229.1
Species Human
Expression System Baculovirus-Insect Cells
Tag His,GST
Accession Number NP_001777.1&NP_001229.1
Synonyms P34CDC2 & CCNE1, CDC2, CDKN1, CDK1, CCNE, CDC28A
Construction A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.
Protein Purity > 90 % as determined by SDS-PAGE
Molecular Weight Approxiamtely 109.7 kDa
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.

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Keywords

CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) P34CDC2 & CCNE1 CDKN-1 CDC 2 CDK 1 CDC2 CDC-2 CDK-1 CDKN1 CDK1 CCNE CDKN 1 CDC28A recombinant recombinant-proteins proteins protein

 

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