CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04450

CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 109.7 kDa and the accession number is NP_001777.1&NP_001229.1.

CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04450

Pack Size	Price	Availability
5 μg	$93	7-10 days
10 μg	$152	7-10 days
20 μg	$248	7-10 days
50 μg	$577	7-10 days

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Product Information

Biological Activity	No Kinase Activity
Description	CDK1 & CCNE1 Heterodimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 109.7 kDa and the accession number is NP_001777.1&NP_001229.1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	N-GST, N-His
Accession Number	NP_001777.1&NP_001229.1
Synonyms	P34CDC2 & CCNE1,CDKN1,CDK1,CDC28A,CDC2,CCNE
Construction	A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & His
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	109.7 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.