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CD8A & CD8B Heterodimer Protein, Cynomolgus, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 44.27 kDa and the accession number is XP_001092778.2&XP_005575417.1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $600 | 7-10 days | |
1 mg | $5,220 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | CD8A & CD8B Heterodimer Protein, Cynomolgus, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 44.27 kDa and the accession number is XP_001092778.2&XP_005575417.1. |
Species | Cynomolgus |
Expression System | HEK293 Cells |
Tag | C-Flag, C-His |
Accession Number | XP_001092778.2&XP_005575417.1 |
Construction | A DNA sequence encoding the cynomolgus CD8A (XP_001092778.2) (Met42-Asp223) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the cynomolgus CD8B (XP_005575417.1) (Met1-Pro170) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Asn63 & Ser19 |
Protein Purity | > 95 % as determined by SDS-PAGE. |
Molecular Weight | 44.27 kDa (predicted); 33.52 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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