Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1.0 ng/ml of recombinant IL-2, ED 50 for this effect is < 1.5 µg/ml.

CD25/IL2R alpha Protein, Human, Recombinant (N-His) is expressed in HEK293 Cells. The accession number is P01589.

Human

HEK293 Cells

TAC-antigen,Tac antigen,soluble IL-2 receptor,IL-2Rα,IL2RA,CD25 antigen

Glu22-Cys213

> 95% as determined by SDS-PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. The IL-2R is made up of 3 subunits - alpha (α), beta (β) and gamma (γ). The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. The β and γ chains of the IL-2R are members of the type I cytokine receptor family. IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). IL-2 Rα is also known as CD25, p55, and Tac (activated T cell) antigen.