AKT3 Protein, Mouse, Recombinant (aa 106-479)

Name: AKT3 Protein, Mouse, Recombinant (aa 106-479)
Brand: TargetMol
SKU: TMPY-04446
Rating: 4.5 (1 reviews)

(Synonyms: v-akt murine thymoma viral oncogene homolog 3, Nmf350, D930002M15Rik, AI851531) Copy Product Info

Synonyms: v-akt murine thymoma viral oncogene homolog 3, Nmf350, D930002M15Rik, AI851531

Catalog No. TMPY-04446 Copy Product Info

AKT3 Protein, Mouse, Recombinant (aa 106-479) is expressed in Baculovirus insect cells. The predicted molecular weight is 43.4 kDa and the accession number is Q9WUA6-1.

Pack Size	Price	USA Stock	Global Stock
10 µg	$152	7-10 days	7-10 days
20 µg	$248	7-10 days	7-10 days
50 µg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.
Description	AKT3 Protein, Mouse, Recombinant (aa 106-479) is expressed in Baculovirus insect cells. The predicted molecular weight is 43.4 kDa and the accession number is Q9WUA6-1.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q9WUA6-1
Construction	A DNA sequence encoding the AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified. Predicted N terminal: Gly
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	v-akt murine thymoma viral oncogene homolog 3, Nmf350, D930002M15Rik, AI851531
Research Background	v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Chemical Properties

Molecular Weight

43.4 kDa (predicted); 46 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.