AKT3 Protein, Human, Recombinant (GST)

Name: AKT3 Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPY-04553
Price: 93 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: v-akt murine thymoma viral oncogene homolog 3, STK-2, RAC-γ, RAC-PK-γ, RAC-PK-gamma, RAC-gamma, PRKBG, PKB-γ, PKB-GAMMA, PKBG, MPPH2, MPPH) Copy Product Info

Synonyms: v-akt murine thymoma viral oncogene homolog 3, STK-2, RAC-γ, RAC-PK-γ, RAC-PK-gamma, RAC-gamma, PRKBG, PKB-γ, PKB-GAMMA, PKBG, MPPH2, MPPH

Catalog No. TMPY-04553 Copy Product Info

AKT3 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 81 kDa and the accession number is Q9Y243-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$93	-	In Stock
10 µg	$152	-	In Stock
20 µg	$248	7-10 days	7-10 days
50 µg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 90 % as determined by SDS-PAGE

Appearance:Solution

Color:Transparent

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	No Kinase Activity
Description	AKT3 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 81 kDa and the accession number is Q9Y243-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	N-GST
Accession Number	Q9Y243-1
Construction	A DNA sequence encoding the full length of human PKBG (NP_005456.1) (Met 1-Glu 479) was expressed with the GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	v-akt murine thymoma viral oncogene homolog 3, STK-2, RAC-γ, RAC-PK-γ, RAC-PK-gamma, RAC-gamma, PRKBG, PKB-γ, PKB-GAMMA, PKBG, MPPH2, MPPH
Research Background	v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Chemical Properties

Molecular Weight

81 kDa (predicted); 81 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.