Immobilized Human VEGF-C, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9 ng/ml determined by ELISA.

VEGFR3/FLT4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 111.3 kDa and the accession number is P35916-1.

Human

HEK293 Cells

VEGFR-3,VEGFR3,VEGF Receptor 3,PCL,LMPH1A,fms related tyrosine kinase 4,FLT-41,FLT41,FLT-4

Tyr25-Ile776

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.