Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 is a Cy5-conjugated Donkey antibody targeting IgG H&L. Donkey Anti-Rabbit IgG H&L Secondary Antibody-Cy5 can be used in IF,FCM,ICC/IF.

IgG

Rabbit

1. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
2. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
3. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
6. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
7. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHGA Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.
10. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, TMAB-02007C5), DAPI (blue) was used to stain the cell nuclei.

IF: 1:100-1000; FCM: 1:100-1000; ICC/IF: 1:100-1000

Polyclonal

Native Rabbit IgG