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Anti-Wnt11 Polyclonal Antibody

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Catalog No. TMAB-01982

Anti-Wnt11 Polyclonal Antibody is a Rabbit antibody targeting Wnt11. Anti-Wnt11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Wnt11 Polyclonal Antibody

Anti-Wnt11 Polyclonal Antibody

😃Good
Catalog No. TMAB-01982
Anti-Wnt11 Polyclonal Antibody is a Rabbit antibody targeting Wnt11. Anti-Wnt11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Wnt11 Polyclonal Antibody is a Rabbit antibody targeting Wnt11. Anti-Wnt11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugated (TMAB-01982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-Wnt11 (TMAB-01982) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa
3. Sample:
Embryo (Mouse) Lysate at 40 μg
293t (Human) Cell Lysate at 30 μg
Primary: Anti-Wnt11 (TMAB-01982) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted; extracellular space; extracellular matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene ”wingless” and to its vertebrate ortholog, Int-1, a mouse proto-oncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins which have features typical of secreted growth factors. Wnt-11 is expressed in the tips of ureteric buds and in the perichondrium, a stem cell-like layer that surrounds the future bones and directs their growth and regeneration. Wnt-11 activity is required for cells to undergo correct convergent extension movements during gastrulation. Human Wnt-11 is also expressed in the lung mesenchyme, the urorectal septum, the urogenital folds, the labioscrotal swellings, and the cortex of the adrenal gland. Unlike other Wnt family members, Wnt-11 is not expressed in the neuroepithelium of the central nervous system. Wnt-11, along with Wnt-8c, is ex-pressed in the posterior region of the chick embryo in the caudal paraxial mesoderm that underlies the prospective caudal neural plate. The gene which encodes Wnt-11 maps to human chromosome 11q13.5.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Wnt11
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Secreted
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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