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Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2
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Catalog No. TMAB-01973
Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2 is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF,FCM.
Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2
Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01973
Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2 is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Blank control (Black line): HUVEc (Black). Primary Antibody (green line): Rabbit Anti-von Willebrand antigen 2 antibody (TMAB-01973) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (von Willebrand antigen 2) Polyclonal Antibody, Unconjugated (TMAB-01973) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (von Willebrand antigen 2) Polyclonal Antibody, Unconjugated (TMAB-01973) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). |
| Immunogen | KLH conjugated synthetic peptide: human Von Willebrand Factor |
| Antigen Species | Human |
| Gene ID | 7450 |
| Uniprot ID | |
| Biology Area | Angiogenic growth factors,Endothelial Cell Markers,Extrinsic,Regulatory,Platelets,Serum Proteins,Endothelial Markers |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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