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Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2

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Catalog No. TMAB-01971

Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2 is a Rabbit antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2

Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2

😃Good
Catalog No. TMAB-01971
Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2 is a Rabbit antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human,Rat (predicted:Mouse,Chicken,Pig,Cow,Horse,Rabbit)
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Vitamin D Receptor) Polyclonal Antibody, Unconjugated (TMAB-01971) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Vitamin D Receptor) polyclonal Antibody, Unconjugated (TMAB-01971) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Vitamin D Receptor
Antigen Species
Human
Gene ID
7421
Uniprot ID
Biology Area
Vitamin D Receptor,Zinc Finger,Vitamin D Receptor,Nuclear hormone receptors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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