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Anti-TUBA8 Antibody (8N451) is a Mouse antibody targeting TUBA8. Anti-TUBA8 Antibody (8N451) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $427 | 7-10 days |
Description | Anti-TUBA8 Antibody (8N451) is a Mouse antibody targeting TUBA8. Anti-TUBA8 Antibody (8N451) can be used in WB,ICC,IHC,FCM. |
Synonyms | Tubulin alpha-8 chain, Tubulin alpha chain-like 2, Tubulin alpha chain like 2, Tubulin alpha 8 chain, TUBAL2, Tuba 8, TBA8_HUMAN, Alpha-tubulin 8, Alpha tubulin 8 |
Reactivity | Human,Rat |
Verified Activity | 1. Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1,000 dilution.
2. Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues?using anti-TUBA8 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded liver cancer tissues using anti-TUBA8 antibody. Counter stained with hematoxylin. 5. ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:100-500; ICC: 1:100-500; FCM: 1:100-200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
Research Background | Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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