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Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

🥰Excellent
Catalog No. TMAY-02149

Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in WB,IP.

Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

🥰Excellent
Catalog No. TMAY-02149
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in WB,IP.
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Product Introduction

Bioactivity
Description
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in WB,IP.
Ig Type
Monoclonal Rabbit IgG
Clone
7R847
Reactivity
Human
Verified Activity
1. TPH1 was immunoprecipitated using:

-Lane A:0.5 mg Jurkat Whole Cell Lysate.

-Lane B:0.5 mg THP-1 Whole Cell Lysate

-0.5 µL anti-TPH1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

-Primary antibody:

-Anti-TPH1 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution.

-Developed using the DAB staining technique.

-Performed under reducing conditions.

-Predicted band size: 55 kDa.

-Observed band size: 60 kDa.

2. Anti-TPH1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: THP1 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:55 kDa.

-Observed band size:55 kDa(We are unsure as to the identity of these extra bands.)
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1 / TPH1 (rh Tryptophan Hydroxylase 1 / TPH1; TMPY-01959; P17752-1; Ile2-Ile444).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human Tryptophan Hydroxylase 1 / TPH1 Protein (TMPY-01959)
Antigen Species
Human
Biology Area
Neurotransmitter Associated Enzymes
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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