Anti-TREM-1 Polyclonal Antibody 2 is a Rabbit antibody targeting TREM-1. Anti-TREM-1 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Mouse,Rat

1. Sample:
kidney (Mouse) Lysate at 40 μg
Primary: Anti-TREM1 (TMAB-01892) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
2. Sample:
Liver (Rat) Lysate at 40 μg
Primary: Anti-TREM1 (TMAB-01892) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
3. Blank control (black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-TREM1 antibody (TMAB-01892)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse TREM1

Mouse

Macrophage / Inflamm.