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Anti-TNFR2/CD120b/TNFR1B Antibody (1J191) is a Rabbit antibody targeting TNFR2/CD120b/TNFR1B. Anti-TNFR2/CD120b/TNFR1B Antibody (1J191) can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $273 | 7-10 days | |
100 μL | $487 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: IHC-P,IHC-Fr,IF Reactivity: Human (predicted:Mouse,Rat) |
Ig Type | IgG |
Clone | 1J191 |
Reactivity | Human (predicted:Mouse,Rat) |
Verified Activity | 1. Sample: Lane 1: Human THP-1 cell Lysates Lane 2: Human K562 cell Lysates Lane 3: Human U937 cell Lysates Lane 4: Human U87MG cell Lysates Lane 5: Human HUVEC cell Lysates Primary: Anti-TNFR2 (TMAB-01874) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 70 kDa 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | IHC-P,IHC-Fr,IF |
Recommended Dose | IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted and Cell membrane. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008] |
Immunogen | A synthesized peptide: human TNFRSF1B |
Antigen Species | Human |
Gene ID | 7133 |
Uniprot ID |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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