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Anti-TLR5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01858
Anti-TLR5 Polyclonal Antibody is a Rabbit antibody targeting TLR5. Anti-TLR5 Polyclonal Antibody can be used in WB.
Anti-TLR5 Polyclonal Antibody
Anti-TLR5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01858
Anti-TLR5 Polyclonal Antibody is a Rabbit antibody targeting TLR5. Anti-TLR5 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TLR5 Polyclonal Antibody is a Rabbit antibody targeting TLR5. Anti-TLR5 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Liver (Mouse) Lysate at 40 μg Ovary (Mouse) Lysate at 40 μg Primary: Anti-TLR5 (TMAB-01858) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 130 kDa 2. Sample: Raw264.7 (Mouse) Cell Lysate at 30 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-TLR5 (TMAB-01858) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 130 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein (By similarity). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. |
| Immunogen | KLH conjugated synthetic peptide: mouse TLR5 |
| Antigen Species | Mouse |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Receptors,Macrophage / Inflamm.,TLR Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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