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Anti-SUMO4 Antibody (4W157)

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Catalog No. TMAC-03915
Alias SUMO4_HUMAN, SUMO 4, SMT3H4, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 2, Small ubiquitin-related modifier 4, Small ubiquitin-like protein 4, Small ubiquitin like modifier 4 protein, IDDM5, dJ281H8.4

Anti-SUMO4 Antibody (4W157) is a Rabbit antibody targeting SUMO4. Anti-SUMO4 Antibody (4W157) can be used in WB,ICC/IF,IP,FCM.

Anti-SUMO4 Antibody (4W157)

Anti-SUMO4 Antibody (4W157)

😃Good
Catalog No. TMAC-03915Alias SUMO4_HUMAN, SUMO 4, SMT3H4, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 2, Small ubiquitin-related modifier 4, Small ubiquitin-like protein 4, Small ubiquitin like modifier 4 protein, IDDM5, dJ281H8.4
Anti-SUMO4 Antibody (4W157) is a Rabbit antibody targeting SUMO4. Anti-SUMO4 Antibody (4W157) can be used in WB,ICC/IF,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-SUMO4 Antibody (4W157) is a Rabbit antibody targeting SUMO4. Anti-SUMO4 Antibody (4W157) can be used in WB,ICC/IF,IP,FCM.
SynonymsSUMO4_HUMAN, SUMO 4, SMT3H4, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 2, Small ubiquitin-related modifier 4, Small ubiquitin-like protein 4, Small ubiquitin like modifier 4 protein, IDDM5, dJ281H8.4
Ig Type
IgG
Clone
4W157
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: Jurkat.
2. ICC staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining SUMO4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining SUMO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining SUMO4 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of 293 cells with SUMO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2, SUMO-3 and SUMO-4, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Ubiquitin and SUMO proteins utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2, SUMO-3 and SUMO-4 proteins localize to the nucleus. In contrast to the other SUMO proteins, SUMO-4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90, which may impair processing to mature form and conjugation to substrates. It is suggested that defects in the gene that encodes for the SUMO-4 protein may be involved in the pathogenesis of type I diabetes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 11 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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