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Anti-SUMO1 Antibody (3N929)
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Catalog No. TMAC-03914
Alias UBL1, UBL 1, Ubiquitin like protein UBL1, Ubiquitin like protein SMT3C, Ubiquitin Like 1, Ubiquitin homology domain protein PIC1, SUMO-1, SMT3H3, Smt3C, SMT3 suppressor of mif two 3 homolog 1, SMT3 homolog 3, SMT3, Small ubiquitin-like modifier 1, Small ubiquitin related modifier 1, Sentrin 1, Sentrin, SENP2, PIC1, PIC 1, OFC10, GMP1, GMP 1, GAP modifying protein 1, DAP1
Anti-SUMO1 Antibody (3N929) is a Rabbit antibody targeting SUMO1. Anti-SUMO1 Antibody (3N929) can be used in WB,ICC/IF,IHC,IP,FCM,ChIP.
Anti-SUMO1 Antibody (3N929)
Anti-SUMO1 Antibody (3N929)
😃Good
Catalog No. TMAC-03914Alias UBL1, UBL 1, Ubiquitin like protein UBL1, Ubiquitin like protein SMT3C, Ubiquitin Like 1, Ubiquitin homology domain protein PIC1, SUMO-1, SMT3H3, Smt3C, SMT3 suppressor of mif two 3 homolog 1, SMT3 homolog 3, SMT3, Small ubiquitin-like modifier 1, Small ubiquitin related modifier 1, Sentrin 1, Sentrin, SENP2, PIC1, PIC 1, OFC10, GMP1, GMP 1, GAP modifying protein 1, DAP1
Anti-SUMO1 Antibody (3N929) is a Rabbit antibody targeting SUMO1. Anti-SUMO1 Antibody (3N929) can be used in WB,ICC/IF,IHC,IP,FCM,ChIP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-SUMO1 Antibody (3N929) is a Rabbit antibody targeting SUMO1. Anti-SUMO1 Antibody (3N929) can be used in WB,ICC/IF,IHC,IP,FCM,ChIP. |
| Synonyms | UBL1, UBL 1, Ubiquitin like protein UBL1, Ubiquitin like protein SMT3C, Ubiquitin Like 1, Ubiquitin homology domain protein PIC1, SUMO-1, SMT3H3, Smt3C, SMT3 suppressor of mif two 3 homolog 1, SMT3 homolog 3, SMT3, Small ubiquitin-like modifier 1, Small ubiquitin related modifier 1, Sentrin 1, Sentrin, SENP2, PIC1, PIC 1, OFC10, GMP1, GMP 1, GAP modifying protein 1, DAP1 |
| Ig Type | IgG |
| Clone | 3N929 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SUMO-1 on Hela cell lysates using anti-SUMO-1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-SUMO-1 antibody. Counter stained with hematoxylin. 7. ICC staining SUMO-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining SUMO-1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining SUMO-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with SUMO-1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer??s disease. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 12 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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