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Anti-STK31 Polyclonal Antibody

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Catalog No. TMAB-13226

Anti-STK31 Polyclonal Antibody is a Rabbit antibody targeting STK31. Anti-STK31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-STK31 Polyclonal Antibody

Anti-STK31 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-13226
Anti-STK31 Polyclonal Antibody is a Rabbit antibody targeting STK31. Anti-STK31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-STK31 Polyclonal Antibody is a Rabbit antibody targeting STK31. Anti-STK31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-STK31 Polyclonal Antibody, Unconjugated (TMAB-13226) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
MCF-7 (Human) Cell Lysate at 30 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-STK31 (TMAB-13226) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
3. Sample:
Testis (Mouse) Lysate at 40 μg
A431 (Human) Cell Lysate at 30 μg
Primary: Anti-STK31 (TMAB-13226) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityTestis specific.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundSTK31 (Serine/threonine protein kinase 31) is similar to a mouse gene that encodes a putative protein kinase with a tudor domain, and shows testis specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human STK31
Antigen Species
Human
Gene Name
STK31
Gene ID
Protein Name
Serine/threonine-protein kinase 31
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 116 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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