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Anti-SOD1 Antibody (4Y683)
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Catalog No. TMAC-03918
Anti-SOD1 Antibody (4Y683) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (4Y683) can be used in WB,ICC/IF,IHC,FCM.
Anti-SOD1 Antibody (4Y683)
Anti-SOD1 Antibody (4Y683)
😃Good
Catalog No. TMAC-03918
Anti-SOD1 Antibody (4Y683) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (4Y683) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-SOD1 Antibody (4Y683) is a Rabbit antibody targeting SOD1. Anti-SOD1 Antibody (4Y683) can be used in WB,ICC/IF,IHC,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Western blot analysis of SOD1 on MCF-7 cells lysates using anti-SOD1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SOD1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOD1 antibody. Counter stained with hematoxylin. 5. ICC staining SOD1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining SOD1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining SOD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with SOD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer's disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 16 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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