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Anti-SMC3 Antibody (2D413)
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Catalog No. TMAC-03806
Alias wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM
Anti-SMC3 Antibody (2D413) is a Rabbit antibody targeting SMC3. Anti-SMC3 Antibody (2D413) can be used in WB,ICC/IF,IHC,FCM.
Anti-SMC3 Antibody (2D413)
Anti-SMC3 Antibody (2D413)
😃Good
Catalog No. TMAC-03806Alias wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM
Anti-SMC3 Antibody (2D413) is a Rabbit antibody targeting SMC3. Anti-SMC3 Antibody (2D413) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-SMC3 Antibody (2D413) is a Rabbit antibody targeting SMC3. Anti-SMC3 Antibody (2D413) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control: Line1: HepG2, Line2: NIH-3T3, Line3:PC12. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 6. ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 141 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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