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Anti-RF1 Antibody (8B803)

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Catalog No. TMAC-03589

Anti-RF1 Antibody (8B803) is a Mouse antibody targeting RF1. Anti-RF1 Antibody (8B803) can be used in WB,FCM.

Anti-RF1 Antibody (8B803)

Anti-RF1 Antibody (8B803)

😃Good
Catalog No. TMAC-03589
Anti-RF1 Antibody (8B803) is a Mouse antibody targeting RF1. Anti-RF1 Antibody (8B803) can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4267-10 days
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Product Introduction

Bioactivity
Description
Anti-RF1 Antibody (8B803) is a Mouse antibody targeting RF1. Anti-RF1 Antibody (8B803) can be used in WB,FCM.
Reactivity
Human
Application
Recommended Dose
WB: 1:500-2000; FCM: 1:100-200
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundTranslation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the "GTP state," it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the "GDP state," it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Antigen Species
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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