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Anti-RF1 Monoclonal Antibody (7R580)

(Synonyms: TB3-1, SUP45L1, ETF1, ERF1, ERF, D5S1995) Copy Product Info
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Synonyms: TB3-1, SUP45L1, ETF1, ERF1, ERF, D5S1995

Catalog No. TMAC-03589 Copy Product Info
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Anti-RF1 Monoclonal Antibody (7R580) is a Mouse antibody targeting RF1. Anti-RF1 Monoclonal Antibody (7R580) can be used in WB,FCM.

Anti-RF1 Monoclonal Antibody
(7R580)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2977-10 days7-10 days
100 µL$4267-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-RF1 Monoclonal Antibody (7R580) is a Mouse antibody targeting RF1. Anti-RF1 Monoclonal Antibody (7R580) can be used in WB,FCM.
SynonymsTB3-1, SUP45L1, ETF1, ERF1, ERF, D5S1995
Ig Type
IgG1
Clone
7R580
Reactivity
Human
Verified Activity
1. Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1,000 dilution. Positive control: Lane1: MCF-7, Lane2: T47D, Lane3: MOLT4, Lane4: Raji.
2. Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
3. Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Anti-RF1 Monoclonal Antibody (7R580),Anti-RF1 Monoclonal Antibody (7R580),Anti-RF1 Monoclonal Antibody (7R580)
Application
Recommended Dose
WB: 1/500-1/2000; FCM: 1/200-1/400
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationPurified by protein A or protein G
AppearanceLiquid
FormulationPBS with 0.05% sodium azide
Concentration1 mg/mL
Research BackgroundThis gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Antigen Species
Human
Gene Name
ETF1
Gene ID
Protein Name
Eukaryotic peptide chain release factor subunit 1
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-RF1 Monoclonal Antibody (7R580) molecular weight