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Synonyms: TB3-1, SUP45L1, ETF1, ERF1, ERF, D5S1995
Anti-RF1 Monoclonal Antibody
(7R580)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $297 | 7-10 days | 7-10 days | |
| 100 µL | $426 | 7-10 days | 7-10 days |
| Description | Anti-RF1 Monoclonal Antibody (7R580) is a Mouse antibody targeting RF1. Anti-RF1 Monoclonal Antibody (7R580) can be used in WB,FCM. |
| Synonyms | TB3-1, SUP45L1, ETF1, ERF1, ERF, D5S1995 |
| Ig Type | IgG1 |
| Clone | 7R580 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1,000 dilution. Positive control: Lane1: MCF-7, Lane2: T47D, Lane3: MOLT4, Lane4: Raji. 2. Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). 3. Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). , ,![]() |
| Application | |
| Recommended Dose | WB: 1/500-1/2000; FCM: 1/200-1/400 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Purified by protein A or protein G |
| Appearance | Liquid |
| Formulation | PBS with 0.05% sodium azide |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. |
| Conjucates | Unconjugated |
| Immunogen | Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli. |
| Antigen Species | Human |
| Gene Name | ETF1 |
| Gene ID | |
| Protein Name | Eukaryotic peptide chain release factor subunit 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 49 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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