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Anti-Rack1 Antibody (8C519)
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Catalog No. TMAC-03499
Alias RACK1, PIG21, HLC-7, H12.3, Gnb2-rs1
Anti-Rack1 Antibody (8C519) is a Rabbit antibody targeting Rack1. Anti-Rack1 Antibody (8C519) can be used in WB,IHC,FCM.
Anti-Rack1 Antibody (8C519)
Anti-Rack1 Antibody (8C519)
😃Good
Catalog No. TMAC-03499Alias RACK1, PIG21, HLC-7, H12.3, Gnb2-rs1
Anti-Rack1 Antibody (8C519) is a Rabbit antibody targeting Rack1. Anti-Rack1 Antibody (8C519) can be used in WB,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Rack1 Antibody (8C519) is a Rabbit antibody targeting Rack1. Anti-Rack1 Antibody (8C519) can be used in WB,IHC,FCM. |
| Synonyms | RACK1, PIG21, HLC-7, H12.3, Gnb2-rs1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2,000 dilution. Positive control: Lane 1: SH-SY-5Y, Lane 2: Rat small intestine. 2. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. Receptor for activated C kinases, termed RACKs, are intracellular receptors for activated PKC that may be involved in the activation-induced translocation of PKC. RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain. The RACK1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana, rice and P.falciparum, and maps to human chromosome 5q35.3. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: C-terminal human RACK1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 35 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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