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Anti-PSGL-1/CD162 Antibody-APC (6E322)
🥰Excellent
Catalog No. TMAY-02647A
Anti-PSGL-1/CD162 Antibody-APC (6E322) is a APC-conjugated Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody-APC (6E322) can be used in FCM.
Anti-PSGL-1/CD162 Antibody-APC (6E322)
Anti-PSGL-1/CD162 Antibody-APC (6E322)
🥰Excellent
Catalog No. TMAY-02647A
Anti-PSGL-1/CD162 Antibody-APC (6E322) is a APC-conjugated Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody-APC (6E322) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PSGL-1/CD162 Antibody-APC (6E322) is a APC-conjugated Mouse antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody-APC (6E322) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6E322 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human SELPLG(CD162) expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human SELPLG(CD162). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSGL-1/CD162 (rh PSGL-1/CD162; TMPY-03531; Q14242-1; Met1-Gly295) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin. |
| Conjucates | APC |
| Others Formats | Unconjugated/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human PSGL-1/CD162 Protein (TMPY-03531) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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