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Anti-PIST Antibody (5T628)
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Catalog No. TMAC-03304
Alias PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL
Anti-PIST Antibody (5T628) is a Rabbit antibody targeting PIST. Anti-PIST Antibody (5T628) can be used in WB,ICC,IF,IP,IHC,FCM.
Anti-PIST Antibody (5T628)
Anti-PIST Antibody (5T628)
😃Good
Catalog No. TMAC-03304Alias PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL
Anti-PIST Antibody (5T628) is a Rabbit antibody targeting PIST. Anti-PIST Antibody (5T628) can be used in WB,ICC,IF,IP,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PIST Antibody (5T628) is a Rabbit antibody targeting PIST. Anti-PIST Antibody (5T628) can be used in WB,ICC,IF,IP,IHC,FCM. |
| Synonyms | PIST, GOPC1, golgi-associated PDZ and coiled-coil motif containing, FIG, dJ94G16.2, CAL |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin. 6. ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | PIST (PDZ protein interacting specifically with TC10), also known as GOPC (golgi associated PDZ and coiled-coil motif containing), CAL or FIG, is a 462 amino acid protein that localizes to the cytoplasm, as well as to the membrane of the golgi apparatus and to the cell junction. Expressed ubiquitously and containing one PDZ (DHR) domain, PIST functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, PIST is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding PIST are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Three isoforms of PIST exist due to alternative splicing events. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human PIST aa 1-100 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 51 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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