Anti-PIK3R1 Antibody (5T395) is a Mouse antibody targeting PIK3R1. Anti-PIK3R1 Antibody (5T395) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

5T395

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIK3R1) Monoclonal Antibody, Unconjugated (TMAB-11445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Jurkat (Human) Cell Lysate at 30 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: Cerebrum (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Heart (Mouse) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Lane 7: Skeletal muscle (Mouse) Lysate at 40 μg
Lane 8: Skeletal muscle (Rat) Lysate at 40 μg
Lane 9: Spleen (Mouse) Lysate at 40 μg
Lane 10: MOLT4 (Human) Cell Lysate at 30 μg
Primary: Anti-PI 3 Kinase p85 alpha (TMAB-11445) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human PI3-kinase p85 subunit alpha protein, around 600-700aa

Human

PIK3R1

Phosphatidylinositol 3-kinase regulatory subunit alpha

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.