Anti-PIBF Polyclonal Antibody is a Rabbit antibody targeting PIBF. Anti-PIBF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PIBF Polyclonal Antibody, Unconjugated (TMAB-11437) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Testis (Mouse) Lysate at 40 μg
Testis (Rat) Lysate at 40 μg
Primary: Anti-PIBF (TMAB-11437) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kD
Observed band size: 83 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PIBF

Human

PIBF1

Progesterone-induced-blocking factor 1

Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect.