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Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody
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Catalog No. TMAC-03307
Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr198). Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody can be used in WB,IP,IF.
Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody
Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody
😃Good
Catalog No. TMAC-03307
Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr198). Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody can be used in WB,IP,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr198). Anti-Phospho-PRKCA (Thr198) Polyclonal Antibody can be used in WB,IP,IF. |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:100-1000; IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Immunogen affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. The phosphorylation at Threonine 198 is cAMP dependent. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A short amino acid sequence containing Thr 198 phosphorylated PKAα cat of human origin |
| Antigen Species | human |
| Molecular Weight | Theoretical: PKAα cat: 40, PKAβ cat: 53, PKAγ cat: 39-40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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