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Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody
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Catalog No. TMAB-01461
Alias p-NFAT2 (Ser233), p-NFAT2 (S233), Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2 (p-Ser233), NFAT2 (p-S233), NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448
Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFAT2 (Ser233). Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody
Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody
😃Good
Catalog No. TMAB-01461Alias p-NFAT2 (Ser233), p-NFAT2 (S233), Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2 (p-Ser233), NFAT2 (p-S233), NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448
Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFAT2 (Ser233). Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFAT2 (Ser233). Anti-Phospho-NFAT2 (Ser233) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | p-NFAT2 (Ser233), p-NFAT2 (S233), Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2 (p-Ser233), NFAT2 (p-S233), NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448 |
| Reactivity | Human,Rat (predicted:Mouse,Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-NFAT2 (Ser233) (Ser235 mouse)(TMAB-01461) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 101 kDa Observed band size: 101 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-NFAT2 (Ser233) (Ser235 mouse)) Polyclonal Antibody, Unconjugated (TMAB-01461) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013] |
| Immunogen | KLH conjugated synthesised phosphopeptide: human NFAT2 around the phosphorylation site of Ser233 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Transcription factors/regulators,NFATs,Other factors,Non-CD,NFATS |
| Molecular Weight | Theoretical: 101 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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