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Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody

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Catalog No. TMAB-01416

Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Ser9). Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody

Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody

😃Good
Catalog No. TMAB-01416
Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Ser9). Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Ser9). Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample: Testis (Rat) Lysate at 30 μg
Primary: Anti-phospho-GSK-3 Beta (Ser9) (TMAB-01416) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 47 kDa
Observed band size: 49 kDa
2. The blue histogram is unstained cells (A549 cells).
The Orange histogram is cells stained with Rabbit IgG/FITC.
The green histogram is cells stained with Rabbit Anti-phospho-GSK-3 Beta (Ser9)/FITC Conjugated antibody (TMAB-01416-FITC).
Isotype control: Cell lines treated with Rabbit IgG/FITC instead of the primary antibody to confirm that primary antibody binding is specific. Concentration: 5 μL in 100 μL 1 X PBS containing 0.5% BSA.
3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, Unconjugated (TMAB-01416) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beta (Ser9)) Polyclonal Antibody, Unconjugated (TMAB-01416 p-GSK-3) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser9) antibody (TMAB-01416)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (TMAB-01416) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-phospho-GSK-3 Beta (Ser9) (TMAB-01416) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human GSK-3 Beta around the phosphorylation site of Ser9
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Integration of energy metabolism,Metabolism of carbohydrates,Diabetes associated,Hypertrophy,Carbohydrate metabolism,Integration of energy,Cancer,Diabetes,Heart disease,Notch Pathway,Other Kinases,Cytoplasmic,Cytoplasmic
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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