Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Ser9). Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Ser9). Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Sample: Testis (Rat) Lysate at 30 μg Primary: Anti-phospho-GSK-3 Beta (Ser9) (TMAB-01416) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution; Predicted band size: 47 kDa Observed band size: 49 kDa 2. The blue histogram is unstained cells (A549 cells). The Orange histogram is cells stained with Rabbit IgG/FITC. The green histogram is cells stained with Rabbit Anti-phospho-GSK-3 Beta (Ser9)/FITC Conjugated antibody (TMAB-01416-FITC). Isotype control: Cell lines treated with Rabbit IgG/FITC instead of the primary antibody to confirm that primary antibody binding is specific. Concentration: 5 μL in 100 μL 1 X PBS containing 0.5% BSA. 3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-GSK-3 Beta (Ser9) Polyclonal Antibody, Unconjugated (TMAB-01416) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beta (Ser9)) Polyclonal Antibody, Unconjugated (TMAB-01416 p-GSK-3) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser9) antibody (TMAB-01416) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 6. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (TMAB-01416) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 7. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-phospho-GSK-3 Beta (Ser9) (TMAB-01416) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Immunogen | KLH conjugated Synthesised phosphopeptide: human GSK-3 Beta around the phosphorylation site of Ser9 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Integration of energy metabolism,Metabolism of carbohydrates,Diabetes associated,Hypertrophy,Carbohydrate metabolism,Integration of energy,Cancer,Diabetes,Heart disease,Notch Pathway,Other Kinases,Cytoplasmic,Cytoplasmic |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.