Anti-PEDF Polyclonal Antibody 2 is a Rabbit antibody targeting PEDF. Anti-PEDF Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Rat)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PEDF) Polyclonal Antibody, Unconjugated (TMAB-10160) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-PEDF (TMAB-10160) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse PEDF

Mouse

SERPINF1

Pigment epithelium-derived factor

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.